Multi-Omics Biomarker Discovery. From Molecules to Markers
Six modular discovery pipelines: cfDNA methylation, metabolomics, proteomics, phosphoproteomics, miRNA, and lipidomics, unified by a computational integration engine that transforms individual hits into validated biomarker panels.
Single-omics discovery misses the full picture.
Most biomarker programs interrogate one molecular layer at a time, and most discovery candidates fail validation. The problem isn't the biology. It's the approach.
Single-Omics Blind Spots
A protein biomarker without metabolomic context, or a methylation hit without proteomic validation, captures one dimension of a multi-dimensional disease. Single-layer discovery misses signatures that only emerge at the intersection of molecular levels.
High False-Discovery Rates
Without cross-omics validation, discovery programs generate candidate lists full of statistical artifacts. When a methylation change, a protein change, and a metabolite change all point to the same pathway, that convergence is your signal.
Discovery-Validation Disconnect
Discovery at one CRO. Validation at another. Different platforms, different sample handling, different statistical frameworks. By the time candidates reach validation, half the signal is lost to technical variance.
Modular Pipelines, Integrated Discovery
Choose the discovery pipelines that match your biology. We handle the integration, from differential profiling through multi-omics computational fusion into actionable panels.
Choose Your Pipelines
Select from 6 modular omics discovery pipelines
Start with one or combine for multi-dimensional profiling
Differential Profiling
Condition vs. control cohorts, rigorous statistics
Systematic comparison identifies condition-specific signatures
Multi-Omics Integration
Computational fusion of cross-omics signals
Individual hits become validated, actionable biomarker panels
Six Discovery Pipelines
Each pipeline is a complete discovery workflow, from sample processing through statistical analysis and candidate selection. Engage one or combine for multi-omics coverage.
cfDNA Methylation Profiling
Epigenetic markers from cell-free DNA in circulation
Untargeted Metabolomics
Small molecule signatures via high-resolution mass spectrometry
Differential Proteomics
Protein abundance changes in circulation
Phosphoproteomics
Signaling pathway activity through phosphorylation profiling
Circulating miRNA Profiling
Stable RNA biomarkers from blood, tissue-specific and disease-responsive
Lipidomics
Lipid species alterations across glycerophospholipids, sphingolipids, and more
Multi-Omics Integration
The computational layer that transforms individual omics results into unified, systems-level biomarker panels. This is where NexysBio's offering transcends what single-omics CROs can deliver.
Unsupervised Discovery
Multi-Omics Factor Analysis (MOFA+) and Similarity Network Fusion identify latent biological processes driving variation across omics layers, revealing disease subtypes invisible to single-omics analysis.
Supervised Panel Selection
Multi-omics machine learning with group-aware regularization selects minimal biomarker panels with cross-omics features. Nested cross-validation ensures performance estimates are unbiased.
Pathway Integration
Differential molecules from all layers mapped onto KEGG and Reactome pathways. Consensus pathways perturbed across methylation, protein, and metabolite levels provide biologically grounded biomarker rationale.
From Individual Hits to Actionable Panels
This is where a methylation marker, a circulating protein, and a lipid species converge into a multi-analyte panel with diagnostic power no single molecule can achieve alone.
Disease Applications
Our discovery platform is condition-agnostic. The same modular pipeline architecture applies to any disease area requiring molecular biomarkers.
Oncology
Early detection panels, minimal residual disease monitoring, treatment response biomarkers
Infectious Disease
Host-response signatures, pathogen-specific metabolic fingerprints, immune correlates
Metabolic Disorders
Insulin resistance profiles, lipid metabolism dysregulation, metabolic syndrome panels
Cardiovascular
Atherosclerosis risk markers, cardiac injury biomarkers, endothelial dysfunction signatures
Neurological
Blood-brain barrier leakage markers, neuroinflammation profiles, neurodegeneration signatures
Autoimmune
Autoantibody panels, inflammatory mediator profiles, disease activity monitoring
Engagement Models
Start with a single pipeline or go end-to-end. Our modular architecture lets you scope the engagement to match your biology, your timeline, and your budget.
Discovery Models
Choose the scope that matches your program stage
- Single Module: focused discovery in one omics layer with full statistical analysis
- Multi-Module: two or more pipelines for cross-omics coverage and validation
- Full Integrated: all six pipelines with multi-omics computational integration
- Discovery + Validation: end-to-end from untargeted profiling through targeted confirmation
Validation Pathways
How discovery candidates get confirmed
- Bisulfite PCR: targeted methylation validation at specific CpG loci
- ELISA / Luminex: protein biomarker quantification in independent cohorts
- Targeted MRM: mass spectrometry validation of metabolite and lipid candidates
- RT-qPCR: quantitative validation of miRNA biomarker candidates
Integrated Services
Your biomarker program connects directly to the broader NexysBio platform. Same team, same context, zero transfer delays.
Nano-Drug Formulation
Nanoparticle-based diagnostic delivery. Functionalized particles for biosensor applications and targeted biomarker sampling.
View serviceAntibody Engineering
Generate monoclonal antibodies against your discovered protein biomarkers for ELISA development, diagnostic applications, or therapeutic targeting.
View serviceVaccine Antigen Discovery
Immune biomarker profiling feeds directly into vaccine programs, including host response signatures and correlates of protection.
View serviceEnzyme & Protein Engineering
Engineered enzymes for diagnostic assay development and signal amplification: custom HRP variants, reporter fusions, and optimized detection reagents.
View serviceBio-Digital Solutions
Custom software built by scientists who understand your workflows, from data dashboards to regulatory-compliant systems.
View serviceCell-Based Assay Services
Functional validation of biomarker candidates in cell-based systems: reporter assays, stimulation panels, and mechanistic confirmation.
Ready to discover your biomarkers?
Let's design your multi-omics discovery program, from pipeline selection through integrated biomarker panel delivery. We'll scope the right combination of discovery modules for your condition and timeline.
Or email us directly at info@nexysbio.com