Nano-Drug Formulation Service

Polymer Nanoparticle Formulations. From Drug to Delivery

We start with your compound and match it to the right polymer system. Our microfluidic platform produces characterized nanoparticle formulations at pilot scale, from chemistry matching through in-vivo proof-of-concept.

You have a promising compound. Now what?

Translating a drug molecule into an effective nanoparticle delivery system requires specialized polymer chemistry, microfluidic engineering, and characterization expertise that most research teams don't have in-house.

Polymer Selection Is Trial-and-Error

Most CROs iterate blindly through polymer options. Without systematic drug-polymer compatibility screening, you waste weeks testing incompatible systems before finding one that works, if you find one at all.

Scale-Up Breaks Your Formulation

Bench-top nanoprecipitation rarely translates to pilot scale. Batch-to-batch variability in size, PDI, and drug loading kills promising formulations before they ever reach animal studies.

No Platform Continuity

Outsourcing each step to a different CRO means lost context, repeated optimization, and months of delays. Your formulation team doesn't talk to your assay team doesn't talk to your animal team.

Drug-First, Polymer-Matched

We don't start with a polymer and hope your drug fits. We start with your drug's properties and systematically identify the optimal polymer system.

1

Your Drug

Solubility, LogP, MW, stability, target tissue

We profile everything about your molecule

2

Polymer Screening

6+ polymer systems evaluated for compatibility

Systematic matching, not random guessing

3

Optimal Match

Best polymer system for your specific molecule

Confirmed by encapsulation and release data

Our platform covers standard FDA-approved polymers, diblock copolymers, and custom synthesis through our collaborator network, so we never run out of options.

Our Service Pipeline

A structured, milestone-driven workflow. Each node is a specific scientific activity with defined techniques, methods, and deliverables. Branch nodes show parallel activities that happen alongside the main pipeline.

Modular by Design. Pick What You Need

You don't have to buy the whole pipeline. Need just polymer screening? Just formulation and characterization? Just in-vitro release? Engage for any set of nodes, or the full end-to-end program. We scope to your needs.

1

Drug Molecule Characterization

We begin by profiling your compound's physicochemical properties: solubility across pH ranges, partition coefficient (LogP), molecular weight, thermal stability, and degradation pathways. This data drives every downstream decision.

Techniques & Methods
Solubility panel (pH 2–9)
LogP determination
Thermal stability (DSC)
Forced degradation studies
Deliverables
Drug property reportStability profile
2

Chemistry Matching: Polymer Compatibility Screening

Based on your drug's properties, we screen it against our polymer library, including PLGA, PLA, PCL, PEG-PLGA, chitosan, and diblock copolymers. We evaluate drug-polymer interaction thermodynamics, miscibility, and predicted encapsulation efficiency to identify the 2–3 best polymer systems.

Techniques & Methods
Drug-polymer miscibility assessment
Hansen solubility parameter matching
Interaction thermodynamics modeling
Encapsulation efficiency prediction
Deliverables
Polymer match reportTop 2–3 candidate systemsGo/No-go recommendation
2a

Custom Diblock Copolymer Evaluation

When standard polymers don't provide optimal compatibility, we evaluate custom diblock copolymers through our synthesis collaborators. Tunable block lengths, molecular weights, and linkage chemistry (ester, thiol, amide) give us access to polymer architectures tailored to your molecule.

Techniques & Methods
Block length optimization
Linkage chemistry selection
Custom synthesis coordination
Deliverables
Custom polymer specificationSynthesis timeline
3

Nanoparticle Formulation: Microfluidic Assembly

Using our microfluidic mixing platform, we produce nanoparticle formulations with precise control over flow rates, solvent ratios, and polymer concentrations. We generate 3–5 lead formulations with different size distributions, targeting specific particle diameters for your application.

Techniques & Methods
Microfluidic rapid mixing
Flow rate optimization (0.5–20 mL/min)
Solvent/anti-solvent ratio tuning
Polymer concentration gradient screening
Deliverables
3–5 lead formulationsProcess parameters for each
3a

Size Distribution & Zeta Potential Characterization

Every formulation batch is characterized by dynamic light scattering (DLS) for particle size, polydispersity index (PDI), and zeta potential for surface charge. We target PDI < 0.2 and validate colloidal stability over time.

Techniques & Methods
DLS particle sizing
Polydispersity index (PDI)
Zeta potential measurement
Stability-over-time tracking
Deliverables
Size distribution profilesZeta potential dataStability assessment
3b

MS-Based Drug Loading Quantification

We quantify actual drug loading and encapsulation efficiency using mass spectrometry, not just UV absorbance estimates. LC-MS/MS gives us precise drug content per mg of nanoparticles and total encapsulation efficiency.

Techniques & Methods
LC-MS/MS drug quantification
Encapsulation efficiency calculation
Loading capacity determination
Deliverables
Drug loading (% w/w)Encapsulation efficiency (%)Batch-to-batch reproducibility data
4

In-Vitro Drug Release Profiling

We evaluate your nanoparticle system's drug release kinetics using dialysis-based methods under multiple physiologically relevant conditions: pH 7.4 (plasma), pH 5.5 (endosomal/tumor), and custom conditions matching your target tissue.

Techniques & Methods
Dialysis-based release (pH 7.4, 5.5, custom)
Sink condition validation
Multi-timepoint sampling (0–168h+)
Release kinetics fitting (zero-order, Higuchi, Korsmeyer-Peppas)
Deliverables
Release profiles per conditionKinetics model fitRelease mechanism analysis
4a

Cell-Based Functional Assays

We validate that your encapsulated drug retains biological activity. Depending on your compound, we run antiviral (plaque reduction, CPE inhibition), antibacterial (MIC/MBC), or enzyme inhibition assays comparing free drug vs. nanoparticle formulation.

Techniques & Methods
Antiviral: plaque reduction, CPE inhibition
Antibacterial: MIC/MBC, time-kill kinetics
Enzyme inhibition: IC50 determination
Cytotoxicity: MTT/MTS cell viability
Deliverables
Functional activity comparison (free vs. encapsulated)IC50/MIC shift dataCell viability profiles
4b

Accelerated Stability Testing

We assess your lead formulation's stability at 4°C (storage), 25°C (ambient), and 37°C (physiological) over 2–4 weeks. We also evaluate lyophilization feasibility for long-term storage as a dry powder.

Techniques & Methods
Multi-temperature stability (4/25/37°C)
Lyophilization cycle development
Reconstitution characterization
Deliverables
Stability report (size, PDI, drug loading over time)Lyophilization feasibility assessment
5

In-Vivo PK/PD Evaluation

For programs advancing to animal studies, we design and execute pharmacokinetic/pharmacodynamic evaluation with your optimized formulation in rodent models. Plasma drug concentration profiling, organ biodistribution, and preliminary efficacy readouts.

Techniques & Methods
Study design & protocol development
Plasma PK profiling (multiple timepoints)
AUC, Cmax, T1/2 calculations
Organ biodistribution (liver, spleen, kidney, lung, brain)
Deliverables
Full PK profileBiodistribution dataPreliminary efficacy readouts
5a

Efficacy in Disease Models

For programs with established animal disease models, we evaluate nanoparticle formulation efficacy versus free drug, measuring therapeutic endpoints, dose-response relationships, and survival curves as appropriate.

Techniques & Methods
Disease model dosing (IP, IV, SC, oral)
Therapeutic endpoint measurement
Dose-response analysis
Deliverables
Efficacy comparison (NP vs. free drug)Program recommendation report

Technical Capabilities

End-to-end nanoparticle development infrastructure, everything under one roof.

Microfluidic Mixing Platform

Precision nanoparticle assembly with controlled flow rates (0.5–20 mL/min), rapid mixing chambers, and automated parameter control. Produces consistent sub-200nm particles with PDI < 0.2.

DLS & Zeta Potential Analysis

Dynamic light scattering for particle size distribution and polydispersity. Electrophoretic light scattering for zeta potential, predicting colloidal stability and in-vivo behavior.

LC-MS/MS Drug Quantification

Mass spectrometry-based drug loading and encapsulation efficiency, not UV estimates. Precise quantification down to ng/mL levels for accurate loading determination.

Drug Release Kinetics

Dialysis-based release profiling under multiple pH conditions (7.4, 5.5, custom). Kinetics modeling with Higuchi, Korsmeyer-Peppas, and zero-order fitting to understand release mechanisms.

Cell-Based Functional Assays

Antiviral (plaque reduction, CPE), antibacterial (MIC/MBC, time-kill), and enzyme inhibition assays. Compare free drug vs. encapsulated drug to validate retained biological activity.

In-Vivo PK/PD & Biodistribution

Full pharmacokinetic profiling in rodent models: plasma drug levels, AUC, Cmax, T1/2. Organ biodistribution across liver, spleen, kidney, lung, and brain. Preliminary efficacy readouts.

Polymer Platform

Access to FDA-approved biodegradable polymers and custom-synthesized systems. We match the polymer to your drug, not the other way around.

PLGA

Poly(lactic-co-glycolic acid)

FDA-approved, tunable degradation rate (weeks to months) via LA:GA ratio. The gold standard for sustained drug release.

BiodegradableFDA-approvedTunable release

PLA

Poly(lactic acid)

Slower degradation than PLGA, higher crystallinity. Ideal for extended release applications requiring months-long drug delivery.

Extended releaseHigh crystallinityStable

PCL

Polycaprolactone

Very slow degradation (1–2 years), excellent flexibility and biocompatibility. Suitable for long-term implantable or injectable depot formulations.

Long-term releaseFlexibleBiocompatible

PEG-PLGA

PEGylated PLGA

PEG corona provides stealth properties: extended circulation time and reduced immune clearance. The standard for IV-administered nanoparticles.

StealthLong-circulatingReduced clearance

Chitosan

Chitosan-based systems

Natural polysaccharide with inherent mucoadhesive properties. Positive surface charge enables mucosal absorption. Ideal for oral, nasal, and pulmonary delivery.

MucoadhesiveNaturalCationic

Diblock Copolymers

Custom-synthesized diblock systems

When standard polymers don't match, we access custom diblock copolymers with tunable block length, MW, and linkage chemistry (ester, thiol, amide) through our synthesis collaborator network.

Custom synthesisTunableApplication-specific

Custom Diblock Copolymer Synthesis

When standard polymers don't match your molecule, we design custom diblock copolymers through our synthesis collaborator network. Tunable block length, molecular weight, and linkage chemistry (ester, thiol, amide) for application-specific properties.

IP & Engagement Model

Transparent, fair, industry-standard IP terms. Your drug, your formulation recipe. You own it.

What You Own

Your drug, your recipe. Full IP rights to your formulation

  • Final optimized formulation composition and recipe
  • All characterization data (DLS, zeta, loading, release)
  • In-vivo efficacy and PK/PD results
  • Complete batch records and analytical reports
  • Freedom to manufacture independently or with any CMO

Our Platform

Process know-how that powers reproducible production

  • Microfluidic production parameters and flow-rate optimization data
  • Proprietary mixer configurations and buffer compositions
  • Stabilizer formulations and post-processing conditions
  • Quality control protocols and analytical method validations
  • Available for licensing or continued production partnership

Co-Development Partnerships Available

For select high-potential programs, we offer co-development partnerships with shared risk and milestone-based economics. Ideal for early-stage programs where aligned incentives accelerate development.

Timeline Overview

Feasibility data in weeks, not months. Our structured pipeline delivers actionable results at every milestone.

1

Drug Profiling

Characterize your compound

Weeks 1–2
2

Polymer Match

Screen & select polymers

Weeks 1–2
3

Formulation

Produce & characterize NPs

Weeks 3–4
4

In-Vitro

Release & functional assays

Weeks 5–8
5

In-Vivo

PK/PD & efficacy

Weeks 8–12+

Typical programs reach feasibility data in 2 weeks and characterized formulation in 4 weeks. Full programs including in-vivo complete in 12 weeks.

Integrated Services

Our nanoparticle formulation service plugs directly into the broader NexysBio platform. Same team, same context, zero transfer delays.

Active

Antibody Engineering

Dual-pipeline antibody discovery combining conventional hybridoma and in-silico evolution. Antibody-nanoparticle conjugation bridges both platforms.

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Active

Vaccine Antigen Discovery

Computationally mine pathogen proteomes for protective epitopes, validate in challenge models, and deliver defined antigen candidates. Nanoparticle-antigen formulations bridge both services.

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Active

Biomarker Discovery

Multi-omics biomarker discovery spanning cfDNA methylation, metabolomics, proteomics, phosphoproteomics, miRNA, and lipidomics with computational integration.

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Active

Enzyme & Protein Engineering

ML-guided enzyme evolution and rational protein design. Engineered therapeutic proteins as payloads for nanoparticle delivery systems.

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Active

Bio-Digital Solutions

Custom software built by scientists who understand your workflows, from data dashboards to regulatory-compliant systems.

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Coming Soon

Cell-Based Assay Services

Antiviral, antibacterial, and cytotoxicity testing. We use the same assay systems for nanoparticle functional validation with zero transfer delays.

Have a compound that needs a nanoparticle delivery system?

Let's evaluate your molecule and design the right polymer nanoparticle formulation. Feasibility assessment starts in as little as one week.

Or email us directly at info@nexysbio.com